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  • Title: Yeast display of antibody fragments: a discovery and characterization platform.
    Author: Feldhaus MJ, Siegel RW.
    Journal: J Immunol Methods; 2004 Jul; 290(1-2):69-80. PubMed ID: 15261572.
    Abstract:
    Yeast display of antibody fragments has proven to be an efficient and productive means for directed evolution of single-chain Fv (scFv) antibodies for increased affinity and thermal stability and, more recently, for the display and screening of a non-immune scFv and immune Fab libraries. A major strength of yeast display as a novel antibody discovery platform is the ability to characterize the binding properties, i.e., the affinity and epitope binding characteristics, of a clone without the need for subcloning, expression and purification of the scFv. This review focuses on novel attributes of yeast display for antibody engineering endeavors.
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