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  • Title: Cloning and over-expression of an alkaline protease from Bacillus licheniformis.
    Author: Tang XM, Shen W, Lakay FM, Shao WL, Wang ZX, Prior BA, Zhuge J.
    Journal: Biotechnol Lett; 2004 Jun; 26(12):975-9. PubMed ID: 15269522.
    Abstract:
    The alkaline protease gene, apr, from Bacillus licheniformis 2709 was cloned into a Bacillus shuttle expression vector, pHL, to yield the recombinant plasmid pHL-apr. The pHL-apr was expressed in Bacillus subtilis WB600, yielding a high expression strain BW-016. The amount of alkaline protease produced in the recombinant increased by 65% relative to the original strain. SDS-PAGE analysis indicated a Mr of 30.5 kDa. The amino acid sequence deduced from the DNA sequence analysis revealed a 98% identity to that of Bacillus licheniformis 6816.
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