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  • Title: Optimization of methanol biosynthesis from methane using Methylosinus trichosporium OB3b.
    Author: Lee SG, Goo JH, Kim HG, Oh JI, Kim YM, Kim SW.
    Journal: Biotechnol Lett; 2004 Jun; 26(11):947-50. PubMed ID: 15269546.
    Abstract:
    Methylosinus trichosporium OB3b oxidized methane to methanol in the presence of a high concentration of Cu2+. Further oxidation of methanol to formaldehyde was prevented by adding 200 mM NaCl which acted as a methanol dehydrogenase H inhibitor. The bacterium, 0.6 mg dry cell ml(-1), in methane/air (1:4, v/v) at 25 degrees C in 12.9 mM phosphate buffer (pH 7) containing 20 mM sodium formate and 200 mM NaCl accumulated 7.7 mM methanol over 36 h.
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