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  • Title: [Study on the extraction process for free isofraxidin and hydrolytic condition of combined isofraxidin in Ciwujia with orthogonal design].
    Author: Yuan X, Bi K, Li Q.
    Journal: Zhong Yao Cai; 2004 Mar; 27(3):209-11. PubMed ID: 15272788.
    Abstract:
    OBJECTIVE: To optimize the extraction and hydrolytic condition of isofraxidin in Ciwujia. METHODS: The study was carried out through orthogoral experimental design. The content of isofraxidin was determined as marker to evaluate the effect on the extraction and hydrolytic condition of different process. RESULTS: The optimum extraction condition was 12 times methanol and 3 times for sixty minutes every time. And the optimum hydrolytic condition was that hydrolysis for two hours, ten times amount of 15% sulfuric acid at 100 degrees C.
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