These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Profiling DNA methylation by bisulfite genomic sequencing: problems and solutions.
    Author: Liu L, Wylie RC, Hansen NJ, Andrews LG, Tollefsbol TO.
    Journal: Methods Mol Biol; 2004; 287():169-79. PubMed ID: 15273411.
    Abstract:
    The surge of interest in DNA methylation during the last two decades has triggered an urgent need for an effective method to detect the methylation status of the cytosines in the genome. Bisulfite genomic sequencing is the most attractive choice so far for many laboratories. Various protocols have been established, but difficulties are often encountered, particularly by individuals who have limited experience in this field. This analysis presents a simple protocol that has consistently worked well in our laboratory. Discussions of potential technical problems and corresponding solutions are also included to facilitate the reproducibility of this protocol.
    [Abstract] [Full Text] [Related] [New Search]