These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Real-time PCR-based assay for quantitative determination of methylation status.
    Author: Lehmann U, Kreipe H.
    Journal: Methods Mol Biol; 2004; 287():207-18. PubMed ID: 15273414.
    Abstract:
    The best studied epigenetic modification in mammals is the methylation of cytosine. During the development and progression of malignant neoplasia, a global hypomethylation is often accompanied by a locus-specific increase in methylation. Also, during normal development specific alterations in DNA methylation patterns take place. In recent years it has become clear that in many situations only quantitative changes in methylation levels occur and that the pure qualitative detection of cytosine methylation misses important biological and pathophysiological information. Therefore, several protocols were developed for the quantitative detection of cytosine methylation. Here, we describe a real-time polymerase chain reaction-based assay for the sensitive and precise quantification of methylated and unmethylated alleles after bisulfite treatment of genomic DNA. In addition to providing quantitative methylation data, this methodology is suitable for high-throughput analysis.
    [Abstract] [Full Text] [Related] [New Search]