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  • Title: Gene expression analysis in cartilage by in situ hybridization.
    Author: Mallein-Gerin F, Gouttenoire J.
    Journal: Methods Mol Med; 2004; 100():105-8. PubMed ID: 15280591.
    Abstract:
    In situ hybridization allows detection and localization of specific nucleic acid sequences directly within a cell or tissue. We present an in situ hybridization protocol using double-stranded DNA or single-stranded RNA probes labeled with [32P] to localize and visualize the temporal and spatial distribution of cartilage-characteristic mRNAs. Probes labeled with this high-energy isotope provide good resolution at the tissue level with relatively low background; as a result of the probes that can be obtained that have a higher specificity to emulsion activity, very short exposure times are required.
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