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  • Title: Further studies on the spectrophotometric determination of amprolium.
    Author: Fink DW, deFontenay G, Bonnefille P, Camarade M, Monier C.
    Journal: J AOAC Int; 2004; 87(3):677-80. PubMed ID: 15287667.
    Abstract:
    The official AOAC spectrophotometric analytical method for amprolium in feeds (961.24) is quantitatively selective for the intact drug in the presence of its primary degradation products. Concentrations evaluated included mixtures of the individual degradates in the presence of amprolium, as well as an equimolar mixture of the 2 degradates. Neither compound responds to the amprolium colorimetric derivatization reaction under any conditions, demonstrating that the official method can be used as an analytical technique for demonstrating the stability of amprolium in medicated feeds. Additionally, liquid chromatography conditions have been established to resolve amprolium from its degradation products.
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