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  • Title: Chemo-enzymatic synthesis of N-arachidonoyl glycine.
    Author: Goujard L, Figueroa MC, Villeneuve P.
    Journal: Biotechnol Lett; 2004 Aug; 26(15):1211-6. PubMed ID: 15289676.
    Abstract:
    N-Arachidonoyl glycine was synthesized in a chemo-enzymatic process where glycine tert -butyl ester was acylated by arachidonic acid and the resulted ester was then de-protected to give the final product. Among various lipases tested and chosen for their ability to cleave fatty amides, that from Candida antarctica B gave the best results resulting in a 39% hydrolysis after 24 h. This enzyme was then used for the reverse N-acylation synthesis and gave a 75% product formation after 24 h using methyl ester of arachadonic acid as acyl donor and acetonitrile as solvent. Direct acylation of glycine gave less than 10% yield.
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