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  • Title: A comparison between the use of a redox mediator in solution and of surface modified electrodes in the electrocatalytic oxidation of nicotinamide adenine dinucleotide.
    Author: Antiochia R, Lavagnini I, Pastore P, Magno F.
    Journal: Bioelectrochemistry; 2004 Sep; 64(2):157-63. PubMed ID: 15296789.
    Abstract:
    Cyclic voltammetry was successfully applied to study the oxidation of nicotinamide adenine dinucleotide (NADH) both in homogeneous and heterogeneous phase. The first case was realized with a solution containing p-methylamino-phenolsulphate (MAP) as redox mediator and the diaphorase (DI) from Clostridium kluveri as enzyme while the second one by using both a glassy carbon (GC) and a carbon nanotube paste (CNTP) electrode modified with electrodeposited films derived from 3,4-dihydroxybenzaldehyde (3,4-DHB). Such systems were successively coupled with glucose dehydrogenase (GDH) reaction to realize the redox chain present in glucose biosensors. A critical comparison of the two systems was also reported.
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