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  • Title: Detection of trophic factor activated signaling molecules in cells by a compact fiber-optic sensor.
    Author: Kapoor R, Kaur N, Nishanth ET, Halvorsen SW, Bergey EJ, Prasad PN.
    Journal: Biosens Bioelectron; 2004 Sep 15; 20(2):345-9. PubMed ID: 15308240.
    Abstract:
    This paper describes a highly sensitive method to detect trophic factor activated signaling molecules in cells using a compact fiber optic biosensor. The method is demonstrated by quantitative detection of phosphorylation of signal transducers and activators of transcription 3 (STAT3) in neuroblastoma cells. A single fiber-optic probe based on total internal reflection fluorescence sensing system is used. A 405 nm diode laser is used for evanescent wave excitation of immobilized labelled analyte on the probe surface. A compact charged coupled device (CCD) based spectrometer is used for recording the fluorescence signal. The method is two orders of magnitude more sensitive than the Western blotting technique.
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