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  • Title: Keeling plots for hummingbirds: a method to estimate carbon isotope ratios of respired CO(2) in small vertebrates.
    Author: Carleton SA, Wolf BO, del Rio CM.
    Journal: Oecologia; 2004 Sep; 141(1):1-6. PubMed ID: 15309607.
    Abstract:
    The carbon isotope composition of an animal's breath reveals the composition of the nutrients that it catabolizes for energy. Here we describe the use of Keeling plots, a method widely applied in ecosystem ecology, to measure the delta(13)C of respired CO(2) of small vertebrates. We measured the delta(13)C of Rufous Hummingbirds ( Selasphorus rufus) in the laboratory and of Mourning ( Zenaida macroura) and White-winged ( Z. asiatica) Doves in the field. In the laboratory, when hummingbirds were fed a sucrose based C3 diet, the delta(13)C of respired CO(2) was not significantly different from that of their diet (delta(13)C(C3 diet)). The delta(13)C of respired CO(2) for C3 fasted birds was slightly, albeit significantly, depleted in delta(13)C relative to delta(13)C(C3 diet). Six hours after birds were shifted to a sucrose based C4 diet, the isotopic composition of their breath revealed that birds were catabolizing a mixture of nutrients derived from both the C3 and the C4 diet. In the field, the delta(13)C of respired CO(2) from Mourning and White-winged Doves reflected that of their diets: the CAM saguaro cactus ( Carnegeia gigantea) and C3 seeds, respectively. Keeling plots are an easy, effective and inexpensive method to measure delta(13)C of respired CO(2) in the lab and the field.
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