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  • Title: Micropatterns of spores displaying heterologous proteins.
    Author: Park TJ, Lee KB, Lee SJ, Park JP, Lee ZW, Choi SK, Jung HC, Pan JG, Lee SY, Choi IS.
    Journal: J Am Chem Soc; 2004 Sep 01; 126(34):10512-3. PubMed ID: 15327288.
    Abstract:
    Micropatterns of Bacillus thuringiensis spores were generated by a combination of surface chemistry, microcontact printing, and spore surface display technique. The outermost layers of B. thuringiensis spores were engineered to present enhanced green fluorescent protein (EGFP), and the biospecific interaction between biotin and streptavidin was utilized to spatially direct the EGFP-presenting spores onto the micropatterned surfaces. The viability of the micropatterned spores was confirmed by the pattern generation of vegetative cells after the germination.
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