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  • Title: Quantification and stability of salbutamol in human urine.
    Author: Forsdahl G, Gmeiner G.
    Journal: J Sep Sci; 2004 Jan; 27(1-2):110-4. PubMed ID: 15335066.
    Abstract:
    A sensitive method for the quantification of free salbutamol in human urine is described. Sample clean up is performed using SPE on a mixed phase extraction column. Derivatisation is performed with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) and the extract is analysed by GC-MS. The method was found to be suitable for use in the doping field, where a cut-off limit of 1 microg salbutamol/mL urine is set by the International Olympic Committee (IOC) and approved by the World Anti-Doping Agency (WADA). Above that value a doping violation occurs. In addition, the stability of salbutamol in human urine has been evaluated.
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