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  • Title: Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus.
    Author: Moravek M, Wegscheider M, Schulz A, Dietrich R, Bürk C, Märtlbauer E.
    Journal: FEMS Microbiol Lett; 2004 Sep 01; 238(1):107-13. PubMed ID: 15336410.
    Abstract:
    Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allows a fast and easy identification of the respective colonies from blood agar plates. The enterotoxins were transferred from the blood agar medium to a nitrocellulose membrane and the immobilized toxins were probed with monoclonal antibodies. The antibodies 2A3 and 1A8 allowed the specific detection of the B component of HBL and the nheA component of NHE. The assay enabled the reliable identification of HBL expressing colonies and differentiation from NHE producing but HBL negative colonies.
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