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  • Title: DNA-PEG-DNA triblock macromolecules for reagentless DNA detection.
    Author: Immoos CE, Lee SJ, Grinstaff MW.
    Journal: J Am Chem Soc; 2004 Sep 08; 126(35):10814-5. PubMed ID: 15339145.
    Abstract:
    The sandwich assay is the most common design for electrochemical DNA sensors. This assay consists of three individual DNA components: an immobilized capture strand, a target strand, and a probe strand containing a redox-active reporter group. We report a simplified DNA assay where two strands of ssDNA, the capture and probe strands, are linked together via a flexible poly(ethylene glycol) (PEG) spacer forming an ABA triblock macromolecule. We have developed an electrochemical assay where the detection signal arises as a consequence of a large structural change induced upon hybridization with target DNA. In this system, the DNA-PEG-DNA macromolecule folds or wraps around the target DNA, bringing the ferrocene probe in close proximity to the electrode, affording an electrochemical response.
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