These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Immobilization of EcoR1, Pae1 and Lpl1 restriction enzymes].
    Author: Kovalenko NA, Sokolov NN, Cherkasova TA, Vonskiĭ VE, Leĭkin IuA.
    Journal: Bioorg Khim; 1992 Feb; 18(2):210-6. PubMed ID: 1534987.
    Abstract:
    In search for sorbents (silica gels, styrene-divinylbenzene copolymers), for immobilization of some restriction endonucleases, derivatives of trityl-containing silochroms are shown to bind EcoRI, PaeI and LplI endonucleases with the retention of 10-20, 60-70 and 40-60% activity, respectively. The immobilized restriction endonucleases have the unchanged substrate specificity, can be used several times and are stable at storage. Tritylaminopropylsilochrom is suggested to be the sorbent of choice.
    [Abstract] [Full Text] [Related] [New Search]