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  • Title: A novel double coating for microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives.
    Author: Xie J, Chen X, Zhang J, Liu J, Tian J, Chen X, Hu Z.
    Journal: J Pharm Biomed Anal; 2004 Sep 21; 36(1):1-8. PubMed ID: 15351041.
    Abstract:
    A novel double coating (DC) was developed for fast and reproducible microemulsion electrokinetic chromatography (MEEKC), as tested with separation and determination of amino acids using laser-induced fluorescence (LIF) detection after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol. The simple coating is a combination of a removable covalent layer and a dynamic SDS coating. Hexamethyldisilazane was utilized for the covalent layer that can be regenerated on-line. Compared with previous no-coating method, the analysis time was shortened; and the reproducibility of migration times was improved.
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