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  • Title: Combined chemical-enzymic synthesis of a dideoxypentasaccharide for use in a study of the specificity of N-acetyl-glucosaminyltransferase-III.
    Author: Kaur KJ, Hindsgaul O.
    Journal: Carbohydr Res; 1992 Mar 30; 226(2):219-31. PubMed ID: 1535553.
    Abstract:
    The biantennary oligosaccharide glycoside beta-D-GlcpNAc-(1----2)-alpha-D- Manp-(1----3)- [beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)]-beta-D-Manp- OR is a potential substrate for N-acetylglucosaminyltransferases (GlcNAcTs) III-V. The dideoxypentasaccharide glycoside beta-D-GlcpNAc-(1----2)-4- deoxy-alpha-D-lyxo-Hexp-(1----3)- [beta-DGlcpNAc-(1----2)-6-deoxy-alpha-D-Manp-(1----6)] beta-D-Manp-O(CH2)7CH3 (5), where the hydroxyl groups that would be acted on by GlcNAcTs IV and V have been removed, was prepared as a possible specific acceptor for GlcNAcT-III. The strategy involved the chemical synthesis of beta-D-GlcpNAc-(1----2)-4-deoxy-alpha-D-lyxo-Hexp-(1----3)-] 6- deoxy-alpha-D-Manp-(1----6)]-beta-D-Manp-O)CH2)7CH3 and then addition of the last GlcpNAc residue using partially purified GlcNAcT-II from rabbit liver. Preliminary results, using detergent extracts from rat kidney, indicate that 5 is an acceptor for a GlcNAcT whose identity remains to be established.
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