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  • Title: The reaction mechanism of plant peroxidases.
    Author: Longu S, Medda R, Padiglia A, Pedersen JZ, Floris G.
    Journal: Ital J Biochem; 2004 Mar; 53(1):41-5. PubMed ID: 15356961.
    Abstract:
    The catalysis of class III plant peroxidases is described based on the reaction scheme of horseradish peroxidase. The mechanism consists in four distinct steps: (a) binding of peroxide to the heme-Fe(III) to form a very unstable peroxide complex, Compound 0; (b) oxidation of the iron to generate Compound I, a ferryl species with a pi-cation radical in the porphyrin ring; (c) reduction of Compound I by one substrate molecule to produce a substrate radical and another ferryl species, Compound II; (d) reduction of Compound II by a second substrate molecute to release a second substrate radical and regenerate the native enzyme. Under unfavourable conditions some inactive enzyme species can be formed, known as dead-end species. Two calcium ions are normally found in plant peroxidases and appear to be important for the catalytic efficiency.
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