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  • Title: Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography-ultraviolet detection analysis.
    Author: Mateo R, Castells G, Green AJ, Godoy C, Cristòfol C.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2004 Oct 25; 810(2):305-11. PubMed ID: 15380729.
    Abstract:
    Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries were good for protoporphyrin IX and other porphyrins (>79%). Applications of this method showed that porphyrins and biliverdin in birds excreta are mainly of biliary-fecal origin rather than urinary origin. Biliverdin and protoporphyrin IX increased proportionately more than the rest of the porphyrins and coproporphyrin III increased more than coproporphyrin I in the bile of Pb-poisoned mallards.
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