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  • Title: The nifU, nifS and nifV gene products are required for activity of all three nitrogenases of Azotobacter vinelandii.
    Author: Kennedy C, Dean D.
    Journal: Mol Gen Genet; 1992 Feb; 231(3):494-8. PubMed ID: 1538703.
    Abstract:
    Strains with mutations in 23 of the 30 genes and open reading frames in the major nif gene cluster of A. vinelandii were tested for ability to grow on N-free medium with molybdenum (Nif phenotype), with vanadium (Vnf phenotype), or with neither metal present (Anf phenotype). As reported previously, nifE, nifN, nifU, nifS and nifV mutants were Nif- (failed to grow on molybdenum) while nifM mutants were Nif-, Vnf- and Anf-. nifV, nifS, and nifU mutants were found to be unable to grow on medium with or without vanadium, i.e. were Vnf- Anf-. Therefore neither vnf nor anf analogoues of nifU, nifS, nifV or nifM are expected to be present in A. vinelandii.
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