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  • Title: [Susceptibility test to rifampin by using quantitative analysis of Mycobacterium tuberculosis mRNA].
    Author: Lu Y, Zhu LZ, Chu NH, Duan LS, Liang GF.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 2004 Aug; 27(8):549-52. PubMed ID: 15388006.
    Abstract:
    OBJECTIVE: To evaluate the significance of quantitative analysis of Mycobacterium tuberculosis mRNA in the test of susceptibility of Mycobacterium tuberculosis strains to rifampin. METHODS: The susceptibility to rifampin of fifty-three clinical isolated strains was test by the percentage of Mycobacterium tuberculosis 85B mRNA copies before and after the use of rifampin, and 1% and 10% were used as the standards for drug resistance, which was compared with the absolute concentration method. Among them, 29 were rifampin resistant strains and 24 rifampin sensitive strains. RESULTS: When the concentration of rifampin was 1 micro g/ml, and 1% and 10% were used as the standards, the accuracy of quantitative analysis of Mycobacterium tuberculosis mRNA with the absolute concentration method was 70% and 81% respectively, and the sensitivity of quantitative analysis of Mycobacterium tuberculosis mRNA was 100% and 100% respectively, while the specificity of quantitative analysis of Mycobacterium tuberculosis mRNA was 33% and 58%. When the concentration of rifampin was 2 micro g/ml, the accuracy of quantitative analysis of mRNA with the absolute concentration method was 85% and 93% respectively, and the sensitivity was 100% and 97% respectively, while the specificity was 67% and 88% respectively. When the concentration of rifampin was 4 micro g/ml, the accuracy was 93% and 93%, and the sensitivity was 93% and 93%, while the specificity was 92% and 92%. CONCLUSIONS: Quantitative analysis of Mycobacterium tuberculosis mRNA was a rapid, sensitive method in rifampin resistance screening. We suggest that the critical concentration of rifampin be 2 micro g/ml, and the critical proportion of mRNA copy be 10%.
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