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  • Title: Expression, crystallization and preliminary crystallographic analysis of YciE, a stress protein from Escherichia coli.
    Author: Liu D, Zhao Y, Fan X, Sun Y, Fox RO.
    Journal: Acta Crystallogr D Biol Crystallogr; 2004 Oct; 60(Pt 10):1888-9. PubMed ID: 15388941.
    Abstract:
    The stress protein Escherichia coli YciE was overexpressed and purified in three chromatographic steps. Crystals were obtained using PEG 4000 as a precipitant. The YciE protein crystals diffracted to 3.0 A resolution using a rotating-anode X-ray source. The lattice type is rhombohedral, with unit-cell parameters a = b = 64.2, c = 167.9 A, alpha = beta = 90, gamma = 120 degrees. The crystal belongs to space group R32.
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