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  • Title: Biochemical properties of endothelin converting enzyme in renal epithelial cell lines.
    Author: Takada J, Hata M, Okada K, Matsuyama K, Yano M.
    Journal: Biochem Biophys Res Commun; 1992 Feb 14; 182(3):1383-8. PubMed ID: 1540181.
    Abstract:
    This is the first report clearly demonstrating the presence of endothelin (ET) converting enzyme (ECE) in non-vascular cells (renal epithelial cell lines, MDCK and LLC-PK1). ECEs derived from these epithelial cells were very similar to the endothelial ECE in the following biochemical properties: 1) The optimum pH was 7.0; 2) the Km value for big ET-1 was approximately 30 microM; 3) the enzyme was potently inhibited by EDTA, o-phenanthroline and phosphoramidon; and 4) the enzyme did not convert big ET-2 or big ET-3. These data suggest that phosphoramidon-sensitive ECE is involved in the processing of big ET-1 to ET-1 in the renal tubule.
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