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  • Title: Specific binding of nucleotides and NAD+ to Clostridium difficile toxin A.
    Author: Lobban MD, Borriello SP.
    Journal: FEBS Lett; 1992 Feb 24; 298(2-3):185-7. PubMed ID: 1544441.
    Abstract:
    Binding of nucleotides, a tetrapolyphosphate, and NAD+ to purified toxin A of Clostridium difficile was determined by monitoring changes in intrinsic fluorescence following excitation at 280 nm, and recording emissions at 340 nm. Binding was specific for concentrations over the range 5 to 100 microM for ATP, GTP, and their respective non-hydrolysable analogues AMP-PNP and Gpp(NH)p, tetrapolyphosphate and NAD+.
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