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  • Title: [Increased expression of Fas and FasL mRNA in peripheral blood lymphocytes in Vogt-Koyanagi-Harada syndrome].
    Author: Chen L, Yang PZ, Zhou HY, Huang XK, Zhong H, Xie CF.
    Journal: Zhonghua Yan Ke Za Zhi; 2004 Aug; 40(8):507-9. PubMed ID: 15454035.
    Abstract:
    OBJECTIVE: To evaluate the expression of Fas and FasL messenger RNA (mRNA) in peripheral blood lymphocytes and its possible role in the pathogenesis of Vogt-Koyanagi-Harada syndrome. METHODS: Blood samples were obtained from 16 patients with Vogt-Koyanagi-Harada syndrome and 19 healthy individuals from January to June, 2000. Total RNA was extracted from peripheral blood lymphocytes. cDNA was prepared from RNA with oligo d(T)(18) and M-MuLV reverse transcriptase. The expression of Fas mRNA and FasL mRNA in peripheral blood lymphocytes was determined using fluorescent quantitative polymerase chain reaction (FQ-PCR). Amplification was carried out through 32 cycles, 30 seconds denaturation at 93 degrees C, 30 seconds annealing at 58 degrees C, and 45 seconds primer extension at 72 degrees C. The final extension time was 7 minutes. RESULTS: The expression of Fas mRNA on peripheral blood lymphocytes [(1.6 +/- 2.0) x 10(6)] was significantly higher than that of the controls [(5.7 +/- 2.0) x 10(5)] (t = 4.50, P < 0.05). The mRNA expression of FasL on peripheral blood lymphocytes [(1.8 +/- 1.5) x 10(6)] was also higher than that of the controls [(4.8 +/- 3.5) x 10(5)] (t = 9.57, P < 0.05). CONCLUSIONS: A high level of Fas mRNA and FasL mRNA expression in activated peripheral blood lymphocytes is observed in patients with Vogt-Koyanagi-Harada syndrome. These long-persisted activated lymphocytes may be responsible for the perpetuation and recurrence of Vogt-Koyanagi-Harada syndrome.
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