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  • Title: [Subretinal transplantation of genetically modified human retinal pigment epithelium cells delay photoreceptor degeneration and functional deterioration in rats].
    Author: Huang Q, Wu JH, Wang F, Xu P, Xia X, Yi J, Zhao XF.
    Journal: Zhonghua Yan Ke Za Zhi; 2004 Aug; 40(8):552-6. PubMed ID: 15454045.
    Abstract:
    OBJECTIVE: To investigate whether subretinal transplantation of genetically modified human retinal pigment epithelium cells can rescue photoreceptor degeneration in RCS rats. METHODS: A spontaneously derived adult human retinal pigment epithelium (RPE) cell line (CRL-2302) was infected by gfp retrovirus, then transfected by liposome mediated CNTF expression plasmid transfer. Around 1 x 10(5) genetic modified cells were injected to subretinal space of the right eye at 4 - 5 weeks old, the left eye was left without injection or injected with PBS as controls. RESULTS: The GFP expression cells under the retina were observed from 1 to 6 weeks after transplantation. Histologic and ultrastructural assessment demonstrated substantial sparing of photoreceptors and correction of RPE phagocytosis defect. Electrophysiological assessment revealed an increased sensitivity of treated eyes to white light. CONCLUSION: The results demonstrate that potential of genetically modified RPE cells for ultimate application in therapeutic transplantation strategies for human retinal degenerative diseases.
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