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  • Title: Spatial characteristics of cisterna magna in rats and novel technique for puncture with a stereotactic manipulator.
    Author: Lebedev SV, Blinov DV, Petrov SV.
    Journal: Bull Exp Biol Med; 2004 Jun; 137(6):635-8. PubMed ID: 15455105.
    Abstract:
    The actual size and sites for puncture of the cisterna magna in adult Wistar rats were estimated on acrylic models. We proposed a new method for obtaining cerebrospinal fluid from narcotized rats. This method consists in controlled introduction of a needle into the cisterna magna through the skin and/or dura mater above this structure using a stereotactic micromanipulator. Experiments on rats with focal and global cerebral ischemia showed that this technique ensures single and repeated sampling of at least 50 microl liquor not containing blood admixtures.
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