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  • Title: [Amplification test-systems in the diagnosis of hemorrhagic fever with renal syndrome and in studies of viral pathogens].
    Author: Iashina LN, Kuzina II, Malysheva TV, Ivanov LI, Khasanova SS.
    Journal: Vestn Ross Akad Med Nauk; 2004; (8):40-3. PubMed ID: 15455692.
    Abstract:
    The "VectoHantivirus-ampli" test system based on rtPCR was shown as fitting the detection of virus RNA in blood samples of patients with hemorrhagic fever with renal syndrome (HFRS) made at early stages and no later than 7 days from the disease onset. A sequence analysis of viral nucleotide sequences of PCR products produced by the test-system ensured the identification of Hantaviruses (pathogens of HFRS). Two genetic variants of Puumula virus were shown to circulate in the territory of the Bashkortostan Republic; they differ by 10.0-13.8%, one of them is absolutely new. The Hantan virus FE genetic variant was detected in the studied samples from the Khabarovsk Territory.
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