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  • Title: Electrochemical quartz crystal nanobalance to detect solvent displacement by pH-induced conformational changes of proteins at Pt.
    Author: Cosman NP, Roscoe SG.
    Journal: Anal Chem; 2004 Oct 01; 76(19):5945-52. PubMed ID: 15456319.
    Abstract:
    The electrochemical quartz crystal nanobalance (EQCN) techniques of simultaneous measurements of frequency and cyclic voltammetry (CV) were used to investigate protein adsorption behavior resulting from pH-induced conformational changes at the Pt electrode at 298 K. The adsorption behavior of holo- and apo-alpha-lactalbumin was studied in electrolyte solutions of pH < 2, 7.4, and 11. The EQCN frequency measurements did not directly monitor the mass of the adsorbed protein at anodic potentials, but instead, at a potential characteristic of the double layer for platinum, gave a measure of the extent of solvent displacement by the adsorbed protein (i.e., a "footprint"), which correlated well with known pH-induced conformational changes of the protein. Simultaneous CV charge transfer measurements provided information on the number of layers of protein adsorbed to the surface. This ability of the EQCN to detect solvent displacement by protein adsorption is potentially useful for biosensors to detect and to monitor protein conformational changes in the bulk and during the adsorption process. The Langmuir adsorption isotherm provided the Gibbs energy of adsorption, DeltaG(ADS), and showed excellent agreement between the CV and EQCN frequency measurements.
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