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  • Title: Fusion expression of human beta-defensin-2 from multiple joined genes in Escherichia coli.
    Author: Wang F, Fang X, Xu Z, Peng L, Cen P.
    Journal: Prep Biochem Biotechnol; 2004 Aug; 34(3):215-25. PubMed ID: 15461138.
    Abstract:
    Human beta-defensin-2 (hBD-2) is a cysteine-rich cationic low molecular weight antimicrobial peptide, which exhibits a broad range of antimicrobial activity without observed acquired resistance. In this work, multiple copies of the hBD-2 gene were linked in tandem and the expression of the multiple joined genes in two fusion expression system, pET28a(+) and pGEX-4T-2, was examined. Using plasmid pET28a(+) with one, two, and four copies of the hBD-2 gene, the expressed level was relatively low, whereas much higher with plasmid pGEX-4T-2, and the fusion products, most of which in insoluble form, account for approximately 26% of the total insoluble cellular proteins.
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