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  • Title: Production of macrophage inflammatory protein-3alpha in human follicular fluid and cultured granulosa cells.
    Author: Kawano Y, Fukuda J, Nasu K, Nishida M, Narahara H, Miyakawa I.
    Journal: Fertil Steril; 2004 Oct; 82 Suppl 3():1206-11. PubMed ID: 15474097.
    Abstract:
    OBJECTIVE: To investigate the role of macrophage inflammatory protein (MIP)-3alpha in human ovulation. DESIGN: Study of the levels of MIP-3alpha in serum and follicular fluid. The effects of interleukin (IL)-1alpha, IL-1 receptor antagonist (RA), and tumor necrosis factor (TNF)-alpha on the secretion of MIP-3alpha by primary cultured granulosa-lutein cells and an immortalized granulosa cell line (GC1a) were investigated. SETTING: Research laboratory at a university medical school. PATIENT(S): Forty-six patients with sterility undergoing in vitro fertilization and embryo transfer (i.v.f.-ET). INTERVENTION(S): Follicular fluid was obtained from study participants, and granulosa-lutein cells and GC1a were incubated with IL-1alpha, IL-1RA, or TNF-alpha for 4 to 32 hours. MAIN OUTCOME MEASURE(S): The concentration of MIP-3alpha in human follicular fluid was measured and correlated with oocyte maturation. We also cultured granulosa cells and examined the regulation of MIP-3alpha production. The concentrations of MIP-3alpha in the serum, follicular fluid, and culture medium were measured using enzyme-linked immunoabsorbent assay. RESULT(S): Concentrations of MIP-3alpha were significantly higher in the follicular fluid, but it was not detected in the serum. Concentrations of MIP-3alpha were statistically significantly higher in the follicular fluid containing mature oocytes than in follicular fluid containing immature oocytes. The production of MIP-3alpha was markedly increased over the basal level after treatment with IL-1alpha and TNF-alpha in a dose-dependent manner. The stimulatory effect of IL-1alpha was inhibited by IL-1RA. CONCLUSION(S): Our data suggest that MIP-3alpha was present in follicular fluid and correlated with oocyte maturation, and was regulated by IL-1alpha and TNF-alpha. Thus, MIP-3alpha may play an important role in the human preovulatory process.
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