These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Regulation of sulfated glycoprotein-2 (clusterin) messenger ribonucleic acid in the rat epididymis.
    Author: Cyr DG, Robaire B.
    Journal: Endocrinology; 1992 Apr; 130(4):2160-6. PubMed ID: 1547732.
    Abstract:
    Sulfated glycoprotein-2 (SGP-2) is secreted by the principal cells of the caput epididymidis and binds to spermatozoa as they transit through this segment. The regulation of SGP-2 in the epididymis is poorly understood. The objectives of these studies were to determine if SGP-2 messenger RNA (mRNA) concentrations in the epididymis are regulated by testosterone or during postnatal development. Northern blot analysis was done using denatured plasmid containing a complementary DNA insert for rat SGP-2. A single 2.1-kilobase transcript was present throughout the epididymis. SGP-2 mRNA concentrations were highest in the caput followed by the initial segment, the cauda, and the corpus epididymidis. To determine if androgens regulate SGP-2 mRNA concentrations, adult rats were bilaterally orchidectomized and testosterone was replaced for 7 days using steroid-filled capsules measuring 2.5 or 18.6 cm. In the initial segment and the caput epididymidis, neither orchidectomy nor testosterone replacement, at either dose, had any effect on SGP-2 mRNA concentrations. In the corpus and cauda epididymidis, bilateral orchidectomy resulted in a 3.5- and 9.4-fold increase, respectively, in SGP-2 mRNA concentrations, whereas testosterone replacement caused a dose-dependent decrease in SGP-2 mRNA concentrations. Unilateral orchidectomy was done to determine if SGP-2 mRNA concentrations are dependent on testicular factors released in the lumen of the epididymis. In the corpus and the cauda epididymidis, unilateral orchidectomy resulted in elevated SGP-2 mRNA concentrations in the ipsilateral epididymis. There were no changes in SGP-2 mRNA concentrations in the initial segment and caput epididymidis. These results provide complementary evidence that the message for SGP-2 is differentially regulated along the epididymis. During postnatal development SGP-2 mRNA concentrations in the caput-corpus epididymidis increased dramatically between 14 and 21 days as well as between 49 and 63 days. Interestingly, between 28 and 42 days, when serum testosterone concentrations are increasing, there was no change in the concentration of SGP-2 mRNA in the caput-corpus epididymidis. Similar results were observed in the cauda epididymidis with the exception that between 28 and 42 days, there was a dramatic decrease in SGP-2 mRNA in the cauda epididymidis. Together these experiments demonstrate that the regulation of SGP-2 mRNA concentrations is segment specific. In the initial segment and caput epididymidis there is no apparent regulation of SGP-2 by testicular factors,whereas in the corpus and cauda epididymidis testosterone can repress SGP-2 mRNA concentrations.
    [Abstract] [Full Text] [Related] [New Search]