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  • Title: Comparison of lysis of bromelain and papain treated red cells in ADCC assays.
    Author: Kumpel BM, Bakács T.
    Journal: Immunol Lett; 1992 Feb 15; 31(3):237-40. PubMed ID: 1548038.
    Abstract:
    Red cells were pretreated with the proteolytic enzymes bromelain or papain prior to use in antibody-dependent cell-mediated cytotoxicity (ADCC) assays with lymphocytes or peripheral blood mononuclear cells (PBMC) as effector cells. At low concentrations of anti-D or anti-A, lysis of papain-treated cells by lymphocytes was greater than that of bromelain-treated cells. Papain digestion resulted in both greater sensitivity to haemolysis by lymphocytes or PBMC and higher agglutination titres of anti-D-sensitised red cells than bromelain. With anti-A, however, although papain also promoted greater haemolysis, it was slightly less effective at red cell agglutination than bromelain.
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