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  • Title: Improving the expression of mini-proinsulin in Pichia pastoris.
    Author: País-Chanfrau JM, García Y, Licor L, Besada V, Castellanos-Serra L, Cabello CI, Hernández L, Mansur M, Plana L, Hidalgo A, Támbara Y, del C Abrahantes-Pérez M, del Toro Y, Valdés J, Martínez E.
    Journal: Biotechnol Lett; 2004 Aug; 26(16):1269-72. PubMed ID: 15483385.
    Abstract:
    Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 degrees C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l(-1) in the culture supernatant after 160 h of growth.
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