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  • Title: Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K.
    Author: Rahman RN, Geok LP, Basri M, Salleh AB.
    Journal: Bioresour Technol; 2005 Mar; 96(4):429-36. PubMed ID: 15491823.
    Abstract:
    The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 degrees C with 37 degrees C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0-10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.
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