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  • Title: Identification of the luciferin-luciferase system and quantification of coelenterazine by mass spectrometry in the deep-sea luminous ostracod Conchoecia pseudodiscophora.
    Author: Oba Y, Tsuduki H, Kato S, Ojika M, Inouye S.
    Journal: Chembiochem; 2004 Nov 05; 5(11):1495-9. PubMed ID: 15515099.
    Abstract:
    The bioluminescence system of the ostracod Conchoecia pseudodiscophora, which is abundant in the Sea of Japan, has been characterized. The luminescence (lambda(max)=463 nm) is produced by a luciferin-luciferase reaction, and the luciferin has been identified as coelenterazine. Coelenterazine, coelenteramide, and coelenteramine from C. pseudodiscophora were quantified by LC-ESI-MS/MS analysis. The coelenterazine content was estimated to be approximately 230 pg per animal by using a calibration curve of synthetic coelenterazine. The reaction between homogenates of C. pseudodiscophora and synthetic coelenterazine showed luminescence activity; this suggests that a coelenterazine-type luciferase is present.
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