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  • Title: [Determination of hyperin in genus Hypericum medicinal plants].
    Author: Wan D, Pei J, Yan Z.
    Journal: Zhong Yao Cai; 2004 Jun; 27(6):397-8. PubMed ID: 15524288.
    Abstract:
    OBJECTIVE: To compare the content of hyperin in Hypericum medicinal plants. METHODS: HPLC method was established. Alltima C18 radial compression column was used with a mobile phase of methanol-0.5% phosphoric acid (45:55, adjusted to pH = 3.0 with triethylamine). The detecting wavelength was 360 nm. Flow rate was 0.8 ml/min, external standard method was quantitative analysis method. RESULTS: The linear range was 0.101-2.023 microg. The average recovery was 96.527%. The result showed that the content of hyperin in H. perforatum, H. forrestii was higher than that of others (1.257% and 0.461% respectively). CONCLUSION: This method provides an accurate and sensitive way in detecting hyperin.
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