These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Modeling the Trypanosoma cruzi Tc85-11 protein and mapping the laminin-binding site.
    Author: Marroquin-Quelopana M, Oyama S, Aguiar Pertinhez T, Spisni A, Aparecida Juliano M, Juliano L, Colli W, Alves MJ.
    Journal: Biochem Biophys Res Commun; 2004 Dec 10; 325(2):612-8. PubMed ID: 15530437.
    Abstract:
    Trypanosoma cruzi expresses a set of glycoproteins encoded by the gp85/trans-sialidase gene superfamily. In this report a structure model is proposed for a cloned member of the superfamily, the Tc85-11 protein. The structure consists of an N-terminus beta-propeller and a C-terminus beta-sandwich interconnected by an alpha-helix. The recombinant protein, corresponding to the N-domain (Tc85-N), binds to laminin in a selective manner. Six synthetic 20-mer peptides from the N-domain adhere onto the surface of LLC-MK(2) cells and two of these peptides specifically inhibit the Tc85-N/laminin interaction, indicating that they are the laminin-binding sites of the molecule. Thus, Tc85-11 and other related members of the family appear to be good candidates to play an important role in T. cruzi infection via a laminin mediated host-parasite interaction.
    [Abstract] [Full Text] [Related] [New Search]