These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Probing molecular interfaces using 2D magic-angle-spinning NMR on protein mixtures with different uniform labeling.
    Author: Etzkorn M, Böckmann A, Lange A, Baldus M.
    Journal: J Am Chem Soc; 2004 Nov 17; 126(45):14746-51. PubMed ID: 15535698.
    Abstract:
    A general NMR strategy to directly study molecular interfaces under magic angle spinning is introduced. The approach is based on the spectroscopic analysis of uniformly, but heterogeneously, labeled molecular mixtures containing the spin species X and Y (X:Y). For the case of an ((15)N:(13)C) labeled sample, the use of NC, NHC, and NHHC transfers is demonstrated. Applied to the ((13)C:(15)N) labeled dimeric form of the 85 amino acid protein Crh, the NHHC approach reveals a variety of monomer-monomer interactions in the microcrystalline state.
    [Abstract] [Full Text] [Related] [New Search]