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  • Title: Cloning and expression of Taxus acyltransferase cDNA.
    Author: Tu J, Zhu P, Cheng KD, Liu J.
    Journal: Z Naturforsch C J Biosci; 2004; 59(9-10):755-61. PubMed ID: 15540611.
    Abstract:
    A new full-length acyltransferase cDNA was obtained from Taxus chinensis by homology-based cloning strategy. The cDNA has an open-reading frame of 1,275 nucleotides, which encodes 425 amino acids with a calculated molecular weight of 47,241 Da and an estimated pI value of 5.93. The deduced amino acid sequence resembles the sequences of other cloned acyltransferases (56-61% identity; 71-75% similarity) involved directly in taxol biosynthetic pathways. This cDNA was expressed in Escherichia coli using the expression vector pET32a(+). The expression band corresponds to the calculated mass plus the N-terminal fusion protein derived from the vector.
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