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  • Title: An RNA ligand inhibits hepatitis C virus NS3 protease and helicase activities.
    Author: Fukuda K, Umehara T, Sekiya S, Kunio K, Hasegawa T, Nishikawa S.
    Journal: Biochem Biophys Res Commun; 2004 Dec 17; 325(3):670-5. PubMed ID: 15541341.
    Abstract:
    The hepatitis C virus non-structural protein 3 (HCV NS3) possesses both protease and helicase activities that are essential for viral replication. In a previous study, we obtained RNA aptamers that specifically and efficiently inhibited NS3 protease activity (G9 aptamers). In order to add helicase-inhibition capability, we attached (U)14 to the 3'-terminal end of a minimized G9 aptamer, DeltaNEO-III. NEO-III-14U was shown to inhibit the NS3 protease activity more efficiently than the original aptamer and, furthermore, to efficiently inhibit the unwinding reaction by NS3 helicase. In addition, NEO-III-14U has the potential to diminish specific interactions between NS3 and the 3'-UTR of HCV-positive and -negative strands. NEO-III-14U showed effective inhibition against NS3 protease in living cells.
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