These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Study on mimotopes of E.coli lipopolysaccharide 2630].
    Author: Luo HB, Zheng SG, Zhu P, Fu N.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2004 Nov; 20(6):682-5. PubMed ID: 15555434.
    Abstract:
    AIM: To screen mimotopes of E.coli lipopolysaccharide(LPS) 2630 from c7c phage display peptide library. METHODS: The LPS mimotopes were screened from c7c phage display peptide library by using affinity chromatograph-purified polyclonal antibody against E.coli LPS 2630(L2630), and the antigenicity of selected clones was identified by ELISA. RESULTS: After 3 rounds of biopanning, 12 out of 20 phage clones were identified as positive clones which could bind to polyclonal anti-L2630 antibody, and 5 of these 12 clones could bind to polyclonal anti-S.typhi LPS 7261(L7261) antibody. The deduced amino acid sequence analysis showed that 8 of 12 clones had the conservative sequence: X-DGLL-XX or X-EDGLL-X. CONCLUSION: The peptides displayed on these phage clones can mimic the epitopes of L2630, and 5 of these phage clones mimic the common epitopes of L2630 and L7261.
    [Abstract] [Full Text] [Related] [New Search]