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  • Title: Simple and rapid procedure suitable for quantitative isolation of low and high molecular weight extracellular nucleic acids.
    Author: Tamkovich SN, Laktionov PP, Rykova EY, Vlassov VV.
    Journal: Nucleosides Nucleotides Nucleic Acids; 2004 Oct; 23(6-7):873-7. PubMed ID: 15560075.
    Abstract:
    The procedure based on binding of nucleic acids with glass surface in presence of chaotropic salts was adapted for efficient isolation of 100-10000 b.p. DNA fragments and 50-10,000 b. RNA fragments. The method provide 90% and 85% efficacy of isolation of 100 b.p. DNA and 100 b. RNA fragments respectively. High molecular weight nucleic acids are isolated with 98% efficacy. Isolated nucleic acids are free from contaminations, influencing nucleic acids modifying enzymes and fluorochromes. The method is rapid, simple and cost-effective.
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