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  • Title: Chiral separation of natural and unnatural amino acid derivatives by micro-HPLC on a Ristocetin A stationary phase.
    Author: Piccinini AM, Schmid MG, Pajpanova T, Pancheva S, Grueva E, Gübitz G.
    Journal: J Biochem Biophys Methods; 2004 Oct 29; 61(1-2):11-21. PubMed ID: 15560918.
    Abstract:
    This paper deals with the chiral separation of Fmoc- and Z-derivatives of natural and unnatural sulfur containing amino acids by micro-HPLC. The separations were carried out in microbore columns packed with a new material based on Ristocetin A bonded to 3.5 microm silica gel. The columns were run in the normal phase, polar organic mode as well as in the reversed phase mode, whereby best results were obtained with the reversed-phase mode using mixtures of triethylamine acetate (TEAA) buffer and methanol as mobile phases.
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