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  • Title: Chlorella dichloromethane extract ameliorates NO production and iNOS expression through the down-regulation of NF kappa B activity mediated by suppressed oxidative stress in RAW 264.7 macrophages.
    Author: Park JY, Cho HY, Kim JK, Noh KH, Yang JR, Ahn JM, Lee MO, Song YS.
    Journal: Clin Chim Acta; 2005 Jan; 351(1-2):185-96. PubMed ID: 15563889.
    Abstract:
    BACKGROUND: It has been proposed that chlorella extracts have antioxidative and anti-inflammatory effects. METHODS: RAW 264.7 murine macrophage cell line was preincubated with various concentrations (0-100 mug/ml) of chlorella dichloromethane extract (CDE) and stimulated with lipopolysaccharide (LPS) to induce oxidative stress and inflammation. RESULTS: Treatments of CDE reduced thiobarbituric acid-reactive substances (TBARS) accumulation, enhancing glutathione level and activities of antioxidative enzymes including superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-px), and glutathione reductase in LPS-stimulated macrophages than LPS-only treated cells. Nitric oxide (NO) production was significantly suppressed in a dose-dependent manner (p<0.05) with an IC(50) of 30.5 microg/ml. Treatment of CDE at 50 microg/ml suppressed NO production to 6% of LPS-control. Treatment with CDE suppressed the levels of inducible nitric oxide synthase (iNOS) protein and mRNA expressions. The specific DNA binding activities of nuclear factor kappa B (NF kappa B) on nuclear extracts from CDE treatments were significantly suppressed with an IC(50) of 62.7 mug/ml in a dose-dependent manner. CONCLUSIONS: CDE ameliorates NO production and iNOS expression through the down-regulation of NF kappa B activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages.
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