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  • Title: Quantitative real time PCR of deoxycytidine kinase mRNA by Light Cycler PCR; in relation to enzyme activity.
    Author: Sigmond J, Loves WJ, Kroep JR, Peters GJ.
    Journal: Nucleosides Nucleotides Nucleic Acids; 2004 Oct; 23(8-9):1347-50. PubMed ID: 15571256.
    Abstract:
    Deoxycytidine kinase (dCK) is essential for the phosphorylation of several deoxyribonucleosides and various analogues such as gemcitabine (2',2'-difluorodeoxycytidine). We developed and optimized a sensitive real time Light Cycler (LC) PCR assay for dCK with SYBR green detection. The enzymatic activity measured in the same human xenografts of dCK correlated excellently with dCK mRNA expression levels measured by the LC. This assay can be used for evaluation of dCK expression in tumors.
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