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  • Title: [Determination of luteolin-7-O-glycoside in the herb of Dracocephalum rupestra by HPLC].
    Author: Ren DM, Lou HX, Ma B, Ji M.
    Journal: Zhongguo Zhong Yao Za Zhi; 2004 Sep; 29(9):860-2. PubMed ID: 15575203.
    Abstract:
    OBJECTIVE: To develop a method for determination of luteolin-7-O-glycoside in the roots, stems, leafs, flowers and aerial parts of Dracocephalum rupestra sampled in different seasons. METHOD: The samples were analyzed on an phenomenex C18 column, with mobile phase of methanol-acetonitrile-0.4% phosphoric acid (30:8:62) at flow rate 1.0 mL x min(-1) and detection at wavelength of 350 nm. RESULT: The content of luteolin-7-O-glycoside in different parts of D. rupestra was different maximum in leaves, while minimum in stems. Luteolin-7-O-glycoside in D. rupestra sampled before blossoming was the highest. CONCLUSION: The method simple, accurate and suitable for the quality evaluation of this plant medicine.
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