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  • Title: Chemical characterization of sialyl oligosaccharides in milk of the Japanese black bear, Ursus thibetanus japonicus.
    Author: Urashima T, Nakamura T, Teramoto K, Arai I, Saito T, Komatsu T, Tsubota T.
    Journal: Comp Biochem Physiol B Biochem Mol Biol; 2004 Dec; 139(4):587-95. PubMed ID: 15581791.
    Abstract:
    Sialyl oligosaccharides were separated from two samples of Japanese black bear milk by extraction with chloroform/methanol, gel filtration on Bio Gel P-2, ion exchange chromatography on DEAE-Sephadex A-50 and high-performance liquid chromatography (HPLC) on a TSK gel Amido-80 column. They were characterized by (1)H-NMR spectroscopy. The structures of four sialyl oligosaccharides separated from the milk were the following: Neu5Ac(alpha2-3)Gal(beta1-4)Glc. Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-3)[Gal(alpha1-3)Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-6)]Gal(beta1-4)Glc. Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-3)[Gal(alpha1-3)[Fuc(alpha1-2)]Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-6)]Gal(beta1-4)Glc. Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-3)[Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-6)Gal(beta1-4)Glc.
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